引用本文:谭 兵1,徐向东1,全 真1,姜小良1,杜红飞2,罗春丽2,吴小候1.肝细胞黏附分子调节蛋白激酶C ε对肾癌786-O细胞增殖和迁移的影响[J].重庆医科大学学报,2014,38(6):748~752
肝细胞黏附分子调节蛋白激酶C ε对肾癌786-O细胞增殖和迁移的影响
Effect of hepatocyte cell adhesion molecule regulating protein kinase C ε on proliferation and migration of renal carcinoma 786-O cells
DOI:
中文关键词:  肝细胞黏附分子  蛋白激酶C ε  肾癌  增殖  迁移
英文关键词:hepatocyte cell adhesion molecule  protein kinase C ε  renal cell carcinoma  proliferation  migration
基金项目:
作者单位
谭 兵1,徐向东1,全 真1,姜小良1,杜红飞2,罗春丽2,吴小候1 1. 重庆医科大学附属第一医院泌尿外科重庆 4000162. 重庆医科大学检验医学院 临床检验诊断学教育部重点实验室重庆 400016 
摘要点击次数: 4596
全文下载次数: 1976
中文摘要:
      目的:研究肝细胞黏附分子(hepatocyte cell adhesion molecule,HepaCAM)对肾癌786-O细胞增殖及迁移的影响。方法:以786-O为目的细胞,通过感染HepaCAM腺病毒(Ad-GFP-HepaCAM)和空载病毒(Ad-GFP),Western blot检测各处理组蛋白激酶C ε(protein kinase C ε,PKCε)膜蛋白、浆蛋白和总蛋白的变化,Ad-GFP-HepaCAM和10 μmol/L PKCε特异性转位抑制剂(εV1-2)处理24 h后基质金属蛋白酶(matrix metalloproteinase,MMP)-9和细胞周期蛋白cyclinD1的变化;平板克隆和划痕实验检测Ad-GFP-HepaCAM及εV1-2对786-O增殖和迁移的影响。结果:感染Ad-GFP-HepaCAM后与对照组比较,PKCε膜蛋白降低(F=82.724,P=0.000;P=0.000)而浆蛋白升高(F=537.006,P=0.000;P=0.000),总蛋白无统计学差异(F=0.548,P=0.605;P=0.805)。Ad-GFP-HepaCAM和εV1-2处理后,MMP-9降低(F=33.109,P=0.001,P=0.000;F=96.868,P=0.000,P=0.000),cyclinD1降低(F=562.626,P=0.000,P=0.000;F=159.433,P=0.000,P=0.000)。Ad-GFP-HepaCAM组比εV1-2组克隆形成率降低(F=259.918,P=0.000;P=0.000),迁移率降低(F=550.169,P=0.000;P=0.000)。结论:HepaCAM可能通过阻止PKCε从胞浆转位到胞膜而部分失活来抑制786-O细胞增殖及迁移。
英文摘要:
      Objective:To investigate the effect of re-expression of hepatocyte cell adhesion molecule(HepaCAM) on the proliferation and migration ability of renal carcinoma 786-O cells. Methods:Protein kinase C ε(PKCε) membrane protein level,PKCε cytosolic protein level and total protein level were determined by Western blot after 786-O cell being infected with Ad-GFP-HepaCAM and Ad-GFP. Matrix metalloproteinase(MMP)-9 and cyclinD1 protein levels were also detected by Western blot after being treated with Ad-GFP-HepaCAM and εV1-2(10 μmol/L,24 h). The proliferation ability and migration ability of 786-O cells were analyzed by colony formation assay and wound healing assay,respectively. Results:When compared with those of control group,PKCε membrane protein level was decreased(F=82.724,P=0.000;P=0.000) and cytosolic protein level was increased(F=537.006,P=0.000;P=0.000),but PKCε total protein level was not changed(F=0.548,P=0.605;P=0.805) after being infected with Ad-GFP-HepaCAM. After expo-sure to Ad-GFP-HepaCAM and εV1-2,MMP-9 protein level was down-regulated(F=33.109,P=0.001,P=0.000;F=96.868,P=0.000,P=0.000) and cyclinD1 protein level was also down-regulated(F=562.626,P=0.000,P=0.000;F=159.433,P=0.000,P=0.000) when compared with those of control group. The colony formation rate was decreased(F=259.918,P=0.000;P=0.000) and migration ratio was also reduced(F=550.169,P=0.000;P=0.000) in Ad-GFP-HepaCAM group compared with those of εV1-2 group. Conclu-sion:HepaCAM could inhibit 786-O cell proliferation and mi-gration via blocking PKCε translocating from cytoplasm to membrane and inactivating PKCε partially.
查看全文  查看/发表评论  下载PDF阅读器
关闭
微信关注二维码